Secondary Antibodies
Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies
(44,276)
Mouse Secondary Antibodies
(14,583)
Rabbit Secondary Antibodies
(6,323)
Donkey Secondary Antibodies
(4,321)
Rat Secondary Antibodies
(1,242)
Sheep Secondary Antibodies
(703)
Chicken Secondary Antibodies
(403)
Bovine Secondary Antibodies
(257)
Pig Secondary Antibodies
(77)
Horse Secondary Antibodies
(75)
Alpaca Secondary Antibodies
(62)
Hamster Secondary Antibodies
(57)
Biosimilar Secondary Antibodies
(9)
Human Secondary Antibodies
(7)
Duck Secondary Antibodies
(3)
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| Content And Storage | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
|---|---|
| Target Species | Mouse |
| Host Species | Rat |
| Conjugate | Unconjugated |
| Applications | ELISA |
| Form | Liquid |
| Isotype | IgM |
| Concentration | 1 mg/mL |
| Antigen | Mouse IgM (Heavy chain) |
| Regulatory Status | RUO |
| Purification Method | Affinity chromatography |
| Product Type | Secondary Antibody |
| Formulation | PBS with 0.1% sodium azide |
| Immunogen | Polyclonal mouse IgM. |
| Classification | Monoclonal |
| Primary or Secondary | Secondary |
| Clone | LO-MM-3 |
Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody, R&D Systems™
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Rat Monoclonal Antibody
| Content And Storage | Store the unopened product at 2 - 8degrees C. Do not use past expiration date. Protect from light. |
|---|---|
| Target Species | Mouse |
| Host Species | Rat |
| Conjugate | Fluorescein |
| Applications | Flow Cytometry |
| Isotype | IgG1 |
| Antigen | IgG2b |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Flow Cytometry |
| Formulation | Supplied in a saline solution containing BSA and Sodium Azide. with Sodium Azide |
| Classification | Monoclonal |
| Primary or Secondary | Secondary |
| Clone | 332723 |
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IgM Antibody (II/41), PE/Cy7, Novus Biologicals™
IgM Antibody (II/41), PE/Cy7, Novus Biologicals™
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Rat Monoclonal Secondary Antibody
| Content And Storage | Store at 4C. Do not freeze. |
|---|---|
| Target Species | Mouse |
| Host Species | Rat |
| Conjugate | PE-Cyanine7 |
| Applications | Flow Cytometry |
| Isotype | IgG2a κ |
| Research Discipline | Immunology |
| Concentration | 0.2 mg/mL |
| Antigen | Mouse IgM |
| Gene Symbols | IGHM |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Flow Cytometry |
| Gene Alias | Constant region of heavy chain of IgM, DKFZp686I15196, DKFZp686I15212, FLJ00385, Hepatitis B virus receptor binding protein, Ig mu chain C region, IGHM, Immunoglobin heavy constant mu, Immunoglobulin heavy constant gamma 3, Immunoglobulin heavy mu chain, immunoglobulin mu, Immunoglobulin mu chain, Imunoglobulin heavy chain, MGC104996, MGC52291, mu, -region CH4 membrane bound domain, VH |
| Gene ID (Entrez) | 3507 |
| Formulation | Aqueous buffer, may contain carrier protein/stabilizer (proprietary). with 0.09% Sodium Azide |
| Classification | Monoclonal |
| Immunogen | The immunogen for this antibody was IgM. |
| Primary or Secondary | Secondary |
| Clone | II/41 |
| Content And Storage | Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody. |
|---|---|
| Target Species | Mouse |
| Host Species | Rat |
| Conjugate | RY655 |
| Applications | Flow Cytometry |
| Form | Liquid |
| Isotype | IgG1 κ |
| Concentration | 0.2 mg/mL |
| Regulatory Status | RUO |
| Gene Alias | Igh-7; immunoglobulin heavy chain 7 (heavy chain of IgE) |
| Product Type | Antibody |
| Gene ID (Entrez) | 16020 |
| Formulation | Aqueous buffered solution containing ≤0.09% sodium azide. |
| Classification | Monoclonal |
| Immunogen | Mouse IgE (pooled) |
| Primary or Secondary | Secondary |
| Clone | R35-72 |
Rat anti-Mouse IgG1 Heavy Chain Secondary Antibody (LO-MG1-2), DyLight 650, Novus Biologicals™
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Rat Monoclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Conjugate | DyLight 650 |
| Target Species | Mouse |
| Host Species | Rat |
| Applications | ELISA |
| Form | Purified |
| Isotype | IgG1 |
| Antigen | IgG1 Heavy Chain |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Formulation | 50mM Sodium Borate |
| Classification | Monoclonal |
| Immunogen | Purified mouse IgG1 from BALB/c mice |
| Primary or Secondary | Secondary |
| Clone | LO-MG1-2 |
Rat anti-Mouse IgM Heavy Chain Secondary Antibody (eB121-15F9), DyLight 488, Novus Biologicals™
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Rat Monoclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Target Species | Mouse |
| Host Species | Rat |
| Conjugate | DyLight 488 |
| Applications | Flow Cytometry |
| Form | Purified |
| Isotype | IgG2a κ |
| Research Discipline | B Cell Development and Differentiation Markers, Immunology |
| Antigen | IgM Heavy Chain |
| Regulatory Status | RUO |
| Purification Method | Protein A or G purified |
| Gene Alias | Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain |
| Gene ID (Entrez) | 3507 |
| Formulation | 50mM Sodium Borate |
| Classification | Monoclonal |
| Immunogen | Mouse IgM |
| Primary or Secondary | Secondary |
| Clone | eB121-15F9 |
| Content And Storage | Refrigerate (2°C to 8°C) |
|---|---|
| Target Species | Mouse |
| Host Species | Rat |
| Conjugate | RB780 |
| Applications | Flow Cytometry |
| Isotype | IgG2a κ |
| Concentration | 0.2 mg/mL |
| Antigen | CD326 (Ep-CAM) |
| Regulatory Status | RUO |
| Gene ID (Entrez) | 17075 |
| Formulation | Aqueous buffered solution containing ≤0.09% sodium azide |
| Immunogen | Glycoconjugates from BALB/c mouse-derived TE-71 medullary thymic epithelial cell line |
| Primary or Secondary | Secondary |
| Clone | G8.8 |
| Content And Storage | Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C. Store in the dark. |
|---|---|
| Target Species | Mouse |
| Host Species | Rat |
| Conjugate | FITC |
| Applications | ELISA,Immunohistochemistry (Frozen) |
| Form | Liquid |
| Isotype | IgG κ |
| Concentration | 1 mg/mL |
| Antigen | Mouse IgG2b |
| Regulatory Status | RUO |
| Purification Method | purified |
| Product Type | Secondary Antibody |
| Formulation | PBS with 0.1% sodium azide |
| Immunogen | Immunoglobulins. |
| Classification | Monoclonal |
| Primary or Secondary | Secondary |
| Clone | LO-MG2b-2 |
| Content And Storage | Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody. |
|---|---|
| Target Species | Mouse |
| Host Species | Rat |
| Conjugate | RY655 |
| Applications | Flow Cytometry |
| Form | Liquid |
| Isotype | IgG2a κ |
| Concentration | 0.2 mg/mL |
| Regulatory Status | RUO |
| Gene Alias | Igh-3; gamma2b; Immunoglobulin heavy constant gamma 2B |
| Product Type | Antibody |
| Formulation | Aqueous buffered solution containing ≤0.09% sodium azide. |
| Classification | Monoclonal |
| Immunogen | Pooled Mouse Ig |
| Primary or Secondary | Secondary |
| Clone | R12-3 |
Mouse IgG2A Antibody, R&D Systems™
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Rat Monoclonal Antibody
| Content And Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution. |
|---|---|
| Target Species | Mouse |
| Host Species | Rat |
| Conjugate | Unconjugated |
| Applications | Flow Cytometry |
| Form | Purified |
| Isotype | IgG1 |
| Antigen | IgG2a |
| Regulatory Status | RUO |
| Purification Method | Protein A or G purified from hybridoma culture supernatant |
| Dilution | Flow Cytometry 0.25 ug/10^6 cells |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Immunogen | Cocktail of mouse IgG 1 and IgG 2A |
| Classification | Monoclonal |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. |
| Primary or Secondary | Secondary |
| Test Specificity | The specificity of this antibody was confirmed by direct ELISA on mouse IgG 2A monoclonal antibodies, and it does not cross-react with IgG 1 IgG 2B IgG 3 IgM, IgA, or IgE antibodies. By flow cytometry, this isotype-specific secondary antibody detects primary antibodies of the mouse IgG 2A isotype, and does not detect primary antibodies of mouse IgG 1 IgG 2B or IgG 3 class. |
| Clone | 344701 |
Rat Anti-Mouse IgG2A PE-conjugated Antibody, R&D Systems™
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Rat Monoclonal Antibody has been used in 2 publications
| Content And Storage | Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied. |
|---|---|
| Target Species | Mouse |
| Host Species | Rat |
| Conjugate | R-PE |
| Applications | Flow Cytometry |
| Form | Purified |
| Isotype | IgG1 |
| Antigen | IgG2a |
| Regulatory Status | RUO |
| Purification Method | Protein A or G purified from hybridoma culture supernatant |
| Dilution | Flow Cytometry 10 uL/10^6 cells |
| Formulation | Supplied in a saline solution containing BSA and Sodium Azide. with Sodium Azide |
| Immunogen | Cocktail of mouse IgG 1 and IgG 2A |
| Classification | Monoclonal |
| Primary or Secondary | Secondary |
| Test Specificity | The specificity of this antibody was confirmed by direct ELISA on mouse IgG 2A monoclonal antibodies, and it does not cross-react with IgG 1 IgG 2B IgG 3 IgM, IgA, or IgE antibodies. By flow cytometry, this isotype-specific secondary antibody detects primary antibodies of the mouse IgG 2A isotype, and does not detect primary antibodies of mouse IgG 1 IgG 2B or IgG 3 class. |
| Clone | 344701 |
Goat anti-Rabbit IgG (H+L) Secondary Antibody, Janelia Fluor 549, Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Target Species | Rabbit |
| Host Species | Goat |
| Conjugate | Janelia Fluor 549 |
| Applications | ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Form | Purified |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Immunogen affinity purified |
| Formulation | 50mM Sodium Borate |
| Classification | Polyclonal |
| Immunogen | This Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against rabbit IgG. |
| Primary or Secondary | Secondary |
Rat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 350, Novus Biologicals™
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Rat Polyclonal Antibody
| Content And Storage | Store at 4°C in the dark. |
|---|---|
| Target Species | Rabbit |
| Host Species | Rat |
| Conjugate | DyLight 350 |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Multi-step |
| Formulation | 50 mM Sodium Borate |
| Classification | Polyclonal |
| Immunogen | Rabbit IgG whole molecule |
| Primary or Secondary | Secondary |